Molecular investigation of cytolysin genes among bacterial isolates recovered from pyospermic patients in Hilla City, Iraq

Enterococcus faecalis and Uropathogenic Escherichia coli (UPEC) are the most common causal agents of urinary tract infections (UTI) infertility in humans. They secrete many cytolytic toxins that impact sperm functions spermatogenesis. The current paper aimed to investigate cytolysins among Bacteriospermia associated bacteria pyospermic patients. 110 Seminal fluid swabs were collected from patients (age Mean±SD, 35.5±2.12 years) September 2020 January 2021. All inoculated on UTI chromogenic medium for primary isolation bacteria. Then suspected Es. En. have been confirmed by PCR using uidA ddl genes, respectively. results revealed compile 42.9% bacteriospermia while 25.71%. Results reveal all E. isolates lta, hylA, sta, stb (100%) sheA (96.7%), stx2 (20%), stx1(3.3%). Hyl, cylLS, cyILL genes. study concludes both a set with possible damage function, causing indirect infertility.